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CHASING THE DUST Oceanographic Expedition - LIVE! Diary, short films and school activities!
Tenerife, Canary Islands
12.00 GMT
Saturday 5 Jan 2008
by Eric Achterberg, Chief Scietist (NOCS) 
We will be setting sail in 6 hours time. Everyone is busy getting their scientific equipment prepared and securely fastened so it will not fall over when the ship starts to rolls in heavy weather.
We all arrived on Tenerife on January 2, and have had two hard working days to unpack all our boxes, install and test our instruments. The delivery of the chemicals that we require for our experiments and measurements was somewhat delayed, but thankfully all arrived this morning with just hours to spare. There is only one last delivery of a computer from Southampton that we are now waiting for, before we can sail.
Everyone is keen and ready to go. We have been waiting for this cruise for a year, as our originally planned cruise for January 2007 was cancelled due to problems with Discovery's engines. The weather is very nice, 23°C and not too much wind. A strong contrast to the cold conditions we had in Britain on our departure, but this is not necessarily good news.
Our aim on this cruise is all about chasing dust storms, and over the last 2 weeks there has been little sign of them. But they have been there over the last 3 months, so we are confident that we will encounter at least some of them over the coming 4 weeks.
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At Sea!
15.30 GMT
Monday 7 Jan 2008
Position 24°30 North, 19°38 West
by Eric Achterberg, Chief Scietist (NOCS) 
We have been at sea for 2 days now, and all has been going very well. Seasickness has not really claimed any victims yet, but that may be due the seasickness tablets that some of us need to keep themselves going.
The first few days are always a little tense as the instruments are being set up for experiments and sampling. Little by little it then becomes clear what is malfunctioning and what is missing. Unfortunately by then it is too late! The good news is that most of the equipment is working well, and we have forgotten only a few minor items. In cases like this, we have to be creative and improvise as obtaining chemicals or spare parts is impossible whilst at sea.
Today the sea is quite calm. We have winds of force 3-4 on the Beaufort scale, what really is a pleasant breeze. From Tenerife we have been heading south. We have undertaken sampling at 4 stations so far, and will now be heading west for the next 2-3 days. Our heading is for 27° North, 30° West, into waters with extremely low levels of surface ocean productivity (oligotrophic waters).  As I write this diary entry, we are cruising through waters which are influenced by the upwelling system off north west Africa. The upwelled (deep) water brings nutrients to the surface, sun-lit waters, which stimulate phytoplankton growth and hence the whole marine ecosystem. We will be sailing to the west of this system and will see the influence of the upwelling system diminish, as the ocean surface will become very blue with few planktonic organisms in the surface.
We have a very nice group of young researchers on the ship. There are multiple nationalities represented, so we can all advise the cook on our favourite dish! The technical support on the vessel, and the shipâs crew and officers are also excellent; they are all trying to make sure that this cruise will be a great success. Time to check up on things!
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A day on the RRS Discovery
15.30 GMT
Tuesday 8 Jan 2008
Position 25°09 North, 22°14 West
by Claire Mahaffey, University of Liverpool
Our typical day starts at 5.00am, when the ship comes to a halt and we start our first station of the day at 5.30am. The Principle Scientist, Eric Achterberg, and other seniour scientists, coordinate the operation and plan where the stations will be and what experiments and activities will be performed at each station.

The routine starts.
Claire Mahaffey
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One essential instrument that is deployed at all stations is the CTD Rosette, a cylindrical frame with a range of oceanographic sensors attached, including salinity, temperature and depth sensors (CTD stands for conductivity, from which salinity is derived, temperature, depth), a fluorometer to measure in-situ fluorescence, an oxygen sensor and twenty-four 20 liter Niskin bottles. The rosette is bulky and weighs more than 1 ton! It is lifted from the deck of the ship into the ocean by a crane and a winch from the starboard (right) side of the ship (see ship plan) operated safely by the shipâs crew.
Once the CTD Rosette is in the water, the sensors send data back to the laboratory, allowing oceanographers to look at detailed profiles of properties such as temperature, oxygen and fluorescence in real time. Here is the screen of the profile we saw this morning.
Other instruments deployed at a typical station are a plankton net, marine snow profilers and optical sensors, but you will hear more about these throughout the cruise.
At mid-morning, we finished the first station and head off for the second, planned at 1pm. The ship steams between station at almost full speed of 11 knots (11 nautical miles per hour). Scientists spend the rest of the afternoon and evening collecting more samples while the ship is moving, or analysing already collected samples in the labs on the ship.
Work typically finishes between 8 and 10pm. Social events are sometimes organized in the evenings e.g. the quiz organized last night by Mark.

Living aboard Discovery.
Claire Mahaffey
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Living Conditions
Living conditions are quite comfortable on the RRS Discovery. Although the ship is fairly large, cabins have to be shared between two. In the cabins, as well as a bed, we have a wardrobe, desk and washbasin. A bathroom is shared between two cabins. There is a lounge (with a large selection of movies) and a small library.
After personal space, the next important thing for comfort is the kitchen staff! They are very important on the ship. By the way, the kitchen on a ship is called âthe galleyâ. For breakfast today, there is a choice of cooked English breakfast, including eggs, cereal and fruits. Lunch is a choice of soup, sandwiches, salad and pizza. Dinner is 'A La Carte'. Tonight we had a choice of soup or spring rolls for starters, and Thai curry with chicken or vegetables for our main course, followed by fruit and ice cream for dessert.
There is always plenty of food on research vessels, which is great for keeping up everyoneâs moral high.
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A sample every 30 minutes!
12.10 GMT
Wednesday 9 Jan 2008
Position 26°30 North, 26°10 West
by Ross Holland (NOCS)
As eighth cruise on the RRS Discovery I feel I am in familiar surroundings now! Itâs always enjoyable coming to sea, but this time, unlike most of the other cruises Iâve been on, the weather is a definite bonus! Iâm used to working in much colder waters than these, so Iâm enjoying the sunshine when I can get out of the lab!
I am the Flow Cytometry Technician at the National Oceanography Centre in Southampton and my role is to study some of the very smallest, yet very important forms of life in the ocean - bacteria and the very smallest phytoplankton. People often question what the point is in studying things that are too small to be seen with the naked eye. People sometimes assume that as I develop my career as a biological oceanographer, I will be allowed to study bigger organisms like whales and dolphins! But thatâs not how it works.
Picophytoplankton (tiniest of plankton including autotrophic bacteria) are not only my passion, they are also a whole science in themselves. Though very small, they are vastly numerous that account for a great deal of the biomass in the ocean. Bacteria are the recycling centre for nutrients needed to support life in the oceans. Their size really misrepresents their importance! Many of them are important in taking up carbon dioxide, a greenhouse gas, out of the atmosphere.
As there can be more than a million individual bacteria per millilitre of ocean water (donât let this put you off swimming in the sea â most are harmless!) no other technique provide such an accurate, time efficient count of their abundance and biomass, as flow cytometry, which is my area of expertise.

Indispensable and loyal friend in the lab - the Robot!
Ross Holland
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The flow cytometer counts and identifies individual cells by passing them through a laser beam and detecting how they fluoresce and scatter the laser light. Different groups of phytoplankton and bacteria will scatter the laser light in their own very specific way. This individual signature can be used to calculate rapidly their density in the water, their role in the ecosystem and even the health condition they are in.
Generally the lack in nutrients or stress conditions can deteriorate the health of these tiny organisms. On this particular cruise, I will be making my contribution to see how Saharan dust, and the iron it caries, affects the health state of these tiniest of phytoplankton.
I am making seawater measurements every 30 minutes throughout the whole of the cruise. For a four-week cruise, thatâs almost 1500 measurements! You may think this doesnât leave much time for visiting the well stocked galley and bar, or for sleeping for that matter, but I have a loyal friend that gives me a break. I have a robot, which automatically takes my water samples for me 24 hours a day! Mmmm I wonder whatâs for dinner!
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Cup of tea anyone? (Happy Birthday Uncle Trev!)
22.10 GMT
Thursday 10 Jan 2008
by Polly Hill (PhD student, NOCS)
Today was hot and sunny as usual - itâs hard to believe it is winter! This is my fourth research cruise in two years, yet it is the first time I have sailed aboard a British ship and so itâs a real treat to finally be understood by the shipâs crew! Another bonus is having delicious food to eat all the time; the cooks on the RRS Discovery look after us very well and seem determined for us all to gain at least a stone before we disembark at the end of the cruise. Iâm only too happy to oblige.
I work in the same research group as Ross Holland, Flow Cytometry technician, who made the diary entry yesterday, describing the fascinating work we do with the tiny but so important marine bacteria and phytoplankton. I am a student in the group and it is my job to assess the welfare of these tiny creatures. I am most interested to find out how they like all this dust that is being dumped on them directly from the Sahara. For the purpose I do quite a complicated series of incubations involving radioactive isotope-labelled substances. By using these radioactive isotopes, it is possible to trace how much of a particular nutrient is assimilated by the bacteria, and this tells me whether they are happy and well, or struggling to survive.

Hich tech science on a shoestring budget!
Polly Hill
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Unlike most of the scientists on board, I donât collect my seawater sample in a bottle, but instead use a thermos flask. Obviously, this amuses the others and I am often teased and asked for a cup of tea (being called Polly, this is something I am used to!). It is not very high tech but there is a good reason for using a thermos flask to collect my samples. The same vacuum in the flask that keeps your (and mine!) tea hot, ensures the seawater water sample remains at the same constant temperature, despite the blazing sun. The double walls also keep my bacteria in the dark so that they do not get confused or traumatised on their way to the lab. Â
One of the best things about studying bacteria is that unlike those biologists studying big organisms such as whales or turtles, we donât have to go far to find what weâre looking for. Every millilitre of seawater contains at least a million bacteria! These tiny bugs may be small, but put them all together and they make up around one-third of the Earthâs biomass. So, can you imagine what sort of an influence they can have on the worldâs oceans!
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Catching the Rain
22.10 GMT
Saturday 12 Jan 2008
Position 23° North, 26° West,
Seastate: moderate (2-3 m waves),
Wind: 6 Beaufort,
Weather: sunny, 24°C
by Claire Powell (University of East Anglia)
Earlier today Eric, the Chief Scientist, asked me to make an entry in the cuise diary. I asked him what I should write about, and his answer was about my typical day and the aerosol sampling I do on board the ship. âAll rightâ I said. Except, now that I think about it I donât really have a âtypicalâ day!
On this particular Saturday I was woken up at 9:30 in the morning by the telephone in my cabin. You might not think that this is very early at all, but I don't go to bed until 3:30am, so 9.30 is pretty early. I managed a tired âHello?â after Iâd worked out where the noise was coming from. âHello Claire?â came the reply âdid you know that itâs raining?â

Rain Catchers!
A funnel set up for collecting rain on top of the monkey island â the equipment is mounted and dismounted each time there is a rain event.
Claire Powell, UEA |
At this point may be I should explain that one of my responsibilitieson the cruise is the collection of rainwater samples. As soon as it starts raining, the officers on duty on the bridge will kindly let me know, just in case I happen to be sleeping as was the case.
It turns out that spotting rain showers at sea can be an experience! As there are no hills to obstruct the view, one can enjoy the sight of approaching individual squalls all the way from the horizon. The effect of the light catching the rain as the wind changes direction can be very pretty.
Still not fully awake and in my pijamas, I jumped into some waterproofs and went up to the roof of the bridge, otherwise known as the monkey island. This is the highest spot on the ship (apart from the crowâs nest!) and it is where I set up the rain sampling equipment. Essentially I use two large funnels fitted with bottles underneath to collect the rainwater. No more fancy than the rainwater sampling you may have done at school.
As the chemical analysis we are doing on rainwater is very sensitive, I have to keep the samples ultra clean. This is why I cannot leave the funnels installed all the time, they gather dust and other particles from the air. Unfortunately for me, they need to be installed every time it starts raining and then stored back in the lab until the next rain shower, and that means I need to get up even in the middle of the night.
As I arrived on the monkey island, I was surprised by a strong downpour, which certainly woke me up. I wish I was awake when I was dressing up! It seems I didnât put on proper waterproof trousers. Needless to say within seconds I was absolutely soaked. After a hot shower and a change of clothes, I went back to the bridge to observe the shower. Itâs a good idea when youâre gathering rainfall (or any other kind of scientific data for that matter!) to make as many notes as possible. I note the time and date of the rainfall, the shipâs location and direction and the speed and direction of the wind, as well as what kind of rainshower it is, whether it is the beginning or end of a shower, whether it has been raining previously.
I dismantled my rain funnels about 11 oâclock and carefully put my rain samples in the large walk-in freezer in the shipâs hold. They will be analysed for iron and other nutrients when I get back to the laboratory in the UK. By the time I had finished it was time for lunch (salad and chips, in case you were interested!), which I felt I absolutely deserved.
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Trapping the Dust
22.10 GMT
Saturday 12 Jan 2008
Position 23° North, 26° West,
Seastate: moderate (2-3 m waves),
Wind: 6 Beaufort,
Weather: sunny, 24°C
by Claire Powell (University of East Anglia)
Hi, it's me again! As well as catching rainwater, my other job on the ship is sampling the particles in the air, or otherwise known as aerosol. I do this with two high volume aerosol samplers, or hivols for short. They look a bit like aluminium beehives and work a bit like a vacuum cleaner: air is sucked in through a set of filters which I change every twenty four hours. I find that the best time to change the filters is just after lunch, whilst we are on station and the ship is stationary while the CTD Rosette samples the 4 km of water beneath our feet.
The hivols are really the first instrument that gives us a sign that Saharan dust around us may be increasing. Although I canât analyse the used filters properly on board, just looking at them is enough to see the reddish-brown particles on the white paper filters. Unfortunately due to the rain earlier in the day the air filters are clean â all the dust in the atmosphere had been washed out by the rain. This is a well known effect â have you ever noticed how the sky in a polluted city is a lot clearer after rain? I carefully fold the filters, pack them into bags and freeze them so I can take them back to the laboratory at home for a more thorough analysis.

A hivol sampler secured to the front of the monkey Island. Just like a vacuum cleaner the hivols sucks and filters air particles (Saharan dust included!) out of the atmosphere.
Claire Powell, UEA |
We can also detect the Saharan dust in the atmosphere high above us using a small hand held device called a sun photometer. All I have to do is point at the sun and, provided that there are no clouds, I obtain a scan of all the wavelengths of light reaching me. This tells me the optical depth of the atmosphere, which is a measure of how many particles there are in the atmosphere scattering the light, i.e. how much dust is blown over us by the winds.
In addition to my own work, I take atmospheric gas samples for a group of scientists in Leeds, UK. Collaboration in this way is a good idea: my aerosol sampling doesnât usually take up a lot of time so I can do some sampling for others who cannot come on the cruise. They will then return the favour on some other cruise and everyone benefits that way.
Gas sampling is very simple and involves pumping air from a sampling line on the monkey island into some special gas bottles. It all takes about half an hour and I take samples like this at the same time every day for the duration of the cruise, all 30 days.
The job done, I join some of my colleagues on the aft deck with a cup of tea. It's just like a tea break at the office, except the view is slightly different. The open ocean is the most beautiful shade of aquamarine blue and very, very clear. It's nice just to watch the waves, even if you don't see any wildlife.
After dinner (Saturday night is curry night on the ship!) I retired to my cabin to pen a few words for the cruise diary. By the way out here, there is no internet, no phone (besides the expensive satellite phone), no going down the park for a walk, no news on the radio, no TV and no going out on the town with your friends, so email becomes your only point of reference with the outside world. We have two deliveries of email per day via a satellite link, and when the download occurs, it is a big event! Everyone likes to get email and to keep in touch with the folks back on land.
After that, itâs time to visit the bar. Well, it is Saturday after all! I just hope it doesnât rain too early tomorrow.
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What's on the menu, for phytoplankton?
15.00 GMT
Sunday 13 Jan 2008
Position 18°44 North, 24°56 West,
Seastate: moderate (2-3 m waves), Wind: 6-7 Beaufort, Weather: sunny, 24°C
by Mark Stinchcombe (NOCS)
It is 15:00h (Greenwich Mean Time) as I write this entry to the Discovery diary and I am waiting for the CTD Rosette cast for this afternoon to finish. I am a marine chemist and my job is to measure the nutrients in the oceans. The CTD Rosette with its array of 24 Niskin bottles is how we obtain water samples for my measurements, from the surface all the way to the dark abyss beneath my feet.

FactBox:
Diatoms - what, where, why |
The sunlit surface of our oceans is populated by small, floating single-celled algae that float with the currents, called phytoplankton. Just like plants, they require nutrients to grow, no different from the flowers in your garden. You may even add more nitrate and phosphate to help the plants grow better. The phytoplankton in the oceans also require nitrate and phosphate to help them grow. Certain phytoplankton, diatoms, even need silicate to construct their ornate shells out of pure glass. Unfortunately the waters around the Canary Islands tend to be pretty poor in nutrients (oligotrophic waters).
As light is also essential for growth, phytoplankton are only found in the surface waters to a depth of 150 meters or so, around our area. Below this depth it is too dark for photosynthesis, and phytoplankton cannot remove the nutrients from seawater. Therefore the concentrations of nitrate and phosphate increase with depth as it gets darker and darker.
My job on board is to measure the concentrations of the nutrients nitrate, phosphate and silicate in the seawater and determine whether or not they are sufficient for phytoplankton. What also intrests me is at what depth these nutrients start increasing (i.e. at what depth phytoplankton stop growing!). Ultimately it is the phytoplankton in this relatively shallow surface layer that are at the bottom of the ocean foodweb and sustain the entire ocean ecosystem. In fact, given even the right conditions phytoplankton in the surface can ocean can change climate, so these small guys can have a very big impact!
Even though automated, the routine can be long. I usually start at around 06:30hrs and can finish around 20:00hrs though I do stop for meal times. If I do manage to finish early though I can relax before going to bed by watching a DVD in the rather large TV room. Unfortunately we are too far from land to receive a TV signal so I canât watch the football. Luckily we get the results on the ship via a daily news-sheet or via e-mails from friends and family back home. Keeping in touch with the outside world can be difficult, but regular e-mails help to make sure weâre never too far from home even though we are thousands of miles away.
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Sending a picture to the bottom of the sea 
15.00 GMT
Sunday 14 Jan 2008
Position 17°36 North, 24°17 West (at the Cape Verde Tenatso Time Series Station)
Seastate: moderate (2-3 m waves), Weather: sunny, 24°C
by Cynthia Dumousseaud (NOCS)
We have been sailing for 10 days now and so far everything has been very pleasant. I am sure my colleagues already told you about the very comfortable living conditions aboard. And the weather! It is always a bonus to sail in good weather conditions. I am used to very rough conditions on cruises, when it is sometimes impossible for us to go outside on deck and do our work . So I am re-discovering the Discovery, and how nice it is to take in the sunshine and ocean all around us.

VIDEO:
Waves climbing aboard?
All part of the trip. |
There is a lot of work to do on the cruise, but so far the atmosphere in the lab has been very relaxed. And it is important to see people on board smiling, even when things donât always work out as we want them, especially on expeditions as long as 30 days.
Although this cruise is focused on sampling the surface Atlantic Ocean ocean and the phytoplankton within it , today we get to do some deep ocean sampling, all the way to 1800 meters beneath my feet. And most of us (well, at least me!), are getting very excited. Well, the data and samples are of course interesting, but another reason is that we get to practice our drawing skills.

SQUEEZE! Sending polystyren coffee cups on a return-trip to the ocean depths is a bit of a tradition. 
Cynthia Dumousseaud
UN-SHRINK! |
As we send our instruments deep into the oceanâs belly, they experience a crushing pressure that increases with depth. A good way to demonstrate this is to put a polystyrene coffee cup on the CTD Rosette just before it goes in the water, and then send it to the bottom. Decorating the white cups before sending it to the bottom is a bit of a tradition. As they disappear from sight, the rapidly increasing hydrostatic pressure squeezes all the air out of the soft and spongy polystyrene. The result is that the cups come back very, very small. You can see on the picture the comparison of a cup before and after the deep cast. And this cup only went to 1800 meters! Imagine if it really reached all the way to the bottom!
Of course we also get to do some work! I am a student at the National Oceanography Centre of Southampton, and I am studying the chemistry of dissolved carbon dioxide in seawater. The ocean depths are an important reservoir of CO2 much larger than that in the atmosphere, and it is the surface ocean that mediates how much CO2 is taken out of or added to the atmosphere. Therefore understand how the exchange of gases between atmosphere and ocean affects the chemistry and biology of the ocean, can teach us about the oceans' potential to change climate.
I am measuring two different parameters in my work. The first one is the pH of sea water. A pump brings seawater continuously to my lab from approximately 5 meters depth, and using an automated system I measure the pH every minute. That's a lot of data after a full month at sea!

VIDEO:
When the Ocean Become Acidic |
The second parameter I measure is dissolved inorganic carbon, or DIC for short. Together pH and DIC can tell us a lot about the chemistry and biology in the surface, about the CO2 exchange between atmosphere and ocean, and ultimately how much more anthropogenic CO2 the oceans can absorb before the ecosystem is affected. In its extreme scenario, excess anthropogenic CO2 in the atmosphere will lower the oceansâ pH, a process known as ocean acidification. But may be it would be more fun to watch a film on that!
Bye for now.
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A low catch 
Tuesday 15 January 2008
Position: 16° 53â North, 25° 05â West, in between the Cape Verde Islands!
Weather, overcast, warm
Seastate: moderate (2 m waves)
by Mark Moore (NOCS)
This is my 5th trip on the RRS Discovery and Iâve just worked out that by the end of these four weeks I will have spent a total of 6 months on this ship in total, so, like Ross who did the blog a few days ago, I feel quite familiar with my surroundings!
I am onboard doing research on phytoplankton. Because phytoplankton use photosynthesis in order to extract the energy from sunlight to take up the carbon dioxide they need to grow, they can only live in the upper part of the ocean, down to around 100m or so. Light attenuates rapidly in water, and some of it is absorbed by the phytoplankton cells themselves. Besides light, like all organisms, phytoplankton also need certain nutrients in order to be able to grow. In addition to nitrogen and phosphorous, small amounts of iron are required. As phytoplankton grows in the well lit surface region, they rapidly reduce the concentration of these nutrients. This results in nutrient-poor conditions and consequently, certain regions of the worldâs oceans donât have large amounts of phytoplankton either. These regions are effectively deserts, and we term them âoligotrophicâ. The region we are studying is towards the edge of one such dessert, known as the sub-tropical North Atlantic gyre.

Land means outside contact! Everyone is exercising their texting thumbs for the first time in ten days.
Mark Moore 
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As you may have already heard (and are likely to hear many more times if we actually do find a dust storm!), the concentrations of nutrients in the surface are so low in parts of this region that even small inputs of dust from the atmosphere supply desperately needed nutrients. However, in the case of one of the nutrients, nitrogen, there is another route for it to get from the atmosphere and into the phytoplankton. Generally phytoplankton cannot use atmospheric nitrogen as the nitrogen molecule is too hard to break apart, with the exception of one group of species, which are capable of extracting nitrogen from the atmosphere (really from the air which is dissolved in water) in a process called nitrogen fixation (or diazotrophy).
You already heard from Ross and Polly about the very small organisms which are dominant in our region. Some of the organisms which perform nitrogen fixation are also very small. The most well known nitrogen fixing marine phytoplankton is called Trichodesmium. The individual cells of this organism are known to bind together to form quite large colonies (a few mm across) which are visible to the naked eye and at times can form very obvious slicks on the surface of the ocean. Unlike the bacteria and picophytoplankton which you have heard can be present in concentrations of 100s of thousands or millions per millilitre of seawater, we may only find a few Trichodesmium colonies in every litre of seawater. However, because it can be an important source of nitrogen, the occurence of this organism can be very important for the operation of the ecosystem in these oligotrophic ocean deserts.
Because Trichodesmium colonies are so much bigger and rarer than the single celled phytoplankton, we collect them using nets. However, even by towing a net through many cubic meters of water we may still not find any and unfortunately today was one such day. We were luckier last week so later in the cruise if we find more I will send you some pictures. As well as doing experiments and making measurements on any Trichodesmium we catch, we are also measuring the rate at which nitrogen is fixed by this and other organisms. To do this we perform an incubation experiment where we control all the conditions while a certain organism is examined. In this case, we feed the organisms their favourite meal (nitrogen gas) only to be able to trace it, we label it with an isotope of nitrogen (15N) which is different to that typically found in the ocean. It is almost like giving Trichodesmium colonies a barium milk shake to trace how much free nitrogen they process!
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Finding my sea legs 
Wednesday 16 January 2008
Position: 13° 30North, 25° 49 West
Wind: Force 7 Beaufort
Seastate: Rough (3-3.5 m waves)
by Anna Macey, PhD Student, (NOCS)
I woke up, as I do every day, to the sound of my alarm going off at 05:30am, and as normal the snooze button got pressed a couple of times. After a week and a half at sea my daily routine has definitely been set. The first task of the day is to hunt down my illusive plastic tubing (it keeps mysteriously disappearing. Eric!!!). I use this tubing to collect water from the 20L niskin bottles filled in during the dawn CTD Rosette cast. My next task is to carry two 20 L carboys full of seawater from the CTD Rosette to my work area in the deck lab (thanks are due to Steve and Dave for the occasional helping hand!). I filter this water to collect samples for protein, DNA and RNA analyses back at NOCS. I am interested in the effects of iron availability on phytoplankton physiology. Iron is a fundamental requirement for many cellular processes in the ocean and I will use the samples I collect on this cruise to look at the effect of iron availability on proteins involved in photosynthesis, respiration and nitrogen fixation.

Collecting water from the CTD Rosette for storage and analysis later in the lab on land.
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I am also using two instruments on the cruise called the FRRF (fast repetition rate fluorometer) and FIRe (Fluorescence Induction and Relaxation) systems, which can show me how healthy the microbial cells in my samples are. This is useful as I can work out whether cells are damaged or stressed in any way due to the iron deficiency they experience or even as a result of me filtering them. After the filtering, itâs a mad dash for lunch (the two seem to always coincide). I spent the afternoon analyzing data from the FRRF and FIRe and preparing for another day of filtering on the RRS Discovery.
This cruise is the first time I have been away at sea and thanks to the combination of the friendly group of scientists and crew onboard, the amazing food (I havenât quite made it to the gym yet but I think I might have to before this month is over!) and the weather we have been getting, I am having a fantastic time! Got to go.
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Single cells, global consequences! 
Friday 18th January 2008
Seastate: moderate (1-1.5 m waves), Wind: 4 Beaufort, Weather: sunny 28°C
Position: 12° 30 North, 27° 35 West
by Ruth Airs
Hi! My name is Ruth and I am a chemist from Plymouth Marine Laboratory. I am part part of a three person team on this cruise and our responsibility is to perform incubation experiments on the ship deck. The idea behind incubations experiments is to observe how phytoplankton behaves under variable conditions, be it changing light intensity, nutrients or any other prescribed stress.

Microscopic cells seen from orbit. SeaWiFS 
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Believe it or not, although phytoplankton in the oceans are limited by nutrients (depending on region), or light (depending on depth, or region e.g. in the poles), they can have too much of a good thing. THE key ingredient for photosynthesis, light, can also damage them. And here, around the Cape Verde Islands, with all the beautiful weather we have been having, the sun is beating down on us. What we are trying to prove with our incubations on the deck is that phytoplankton can produce certain compounds to protect themselves from this high intensity sunlight, just like throwing sun lotion on themselves.
Phytoplankton use various ways to protect themselves against light damage, or photo-inhibition. Some secrete shiny calcite shells to that reflect back excess light, other can even repair their damaged proteins. Coccolithophorids example are masters at this. They produce a compound called dimethyl sulphide propionate (DMSP), which is thought to have a number of different functions in the cell. One of those suggested is as an antioxidant to protect against damage from high light intensity. We are particularly interested in explaining how and why DMSP is produced by phytoplankton, because it breaks down to form a gas called dimethyl sulphide (DMS) which can have a significant effect on our climate.

VIDEO:
When the Ocean Become Acidic, what might be impact on the coccolithophore blooms and ocean ecosystems? |
DMS happens to be released when coccolithophorids are exposed to intense light and it also happens to promote the formation of clouds in the atmosphere. The net results is that the clouds could shield the coccolithophorids from the intense light that caused them to release the DMS in the first place. The beauty of it is that something as small and insignificant as a single cellular organism only 5 microns across can alter the atmosphere above it to benefit its own kind!
The consequences however could be more than just a curiosity. Given enough of these single cells, and under stressed conditions, the enhanced cloud formation can significantly alter the global albedo and amount of sunlight that reaches the planetâs surface. The implications for climate long term can be significant, and we are only talking about single celled organisms here!
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Outside it is still dark... 
Saturday 19 January 2008
Position 12 30°North, 30 00°West, southwest of the Cape Verde islands
Seastate: moderate 1-1.5 m, Weather: sunny, 25°C
Haze of low clouds and dust
by David Mckee, NERC Research Fellow, University of Strathclyde
It's just another day on the RRS Discovery and my alarm tells me it is 5.00am and time get the instruments ready for their morning dip. Outside it is still dark, so it has been fun setting up under starry skies, with some southern constellations like Centaurus and Scorpio to keep you company. They are certainly difficult to see from Glasgow! A few of us have also been doing some planet-spotting, with Mercury, Venus, Mars and Jupiter on the observed list. Well, we havenât had any dust-storms yet so the air is crystal clear.
The morning instrument cast is also a good time for animal spotting as the ship is lit up with spotlights which attracts squid and fish. Yesterday I saw my first flying fish. It actually jumped right out of the water and landed on the deck. This one got lucky as I put it back into the ocean, but I am told that they taste pretty good so the next one better watch out!
This cruise is dedicated to understanding the impact of Saharan dust on nutrients and biological productivity in the eastern (sub)-tropical Atlantic Ocean, and it looks like we just might get it soon. My own work is settling into a nice routine. Personally, I am interested in the optical properties of seawater and observing chlorophyll in the oceans both with instruments from ships and satellites in orbit. Dust in the atmosphere can play tricks with satellite observations, so I have to be aware how to correct for it.

Lights Scattering and absorption measurement system is deployed
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Most of my previous work has been in the shallow seas (<200m) around the UK, which are close to land and often there is a lot of suspended material in the water. Particles in the water tend to make the water appear green or even brown in colour. Out here the water is clear deep blue because there is much less suspended material in the water. This means that sunlight can penetrate deeper into the water and less of it is reflected back into space. Despite that, by the time you get down to 100 meters, the red, orange, yellow, and green wavelengths of light are absorbed so that the remaining light we see is composed of the shorter blue and violet wavelengths. To study the absorption of light in the oceans I use optical measuring instruments to obtain vertical profiles of light with depth.
Work for me is usually over by about 20.00h so it is a long day, but it is exciting to be working on a big ship with so many enthusiastic scientists. The time really flies past. Meal times are good for catching up with the other teams to see what they have been finding. Hats off to they guys in the galley too â the food is very good and is certainly abundant!
We would love to get a big dust storm to chase after, but thatâs not up to us. After a couple of weeks I think everyone is missing their families. It should get easier when the end gets closer, as it still seems quite far off at the moment. In the meantime I am enjoying the company of a great bunch of people and trying to make the most of every moment I get on the RRS Discovery.
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Dust! Finally!
14:30 GMT
January 21 2008
Position 12°30 North, 32°38 West
Water depth 4450 m
Sea state: moderate, 2.5 m waves, Force 6 Beaufort
by Eric Achterberg, Chief Scientist
Life on board
We have been at sea now for more than 2 weeks, and are past the halfway point of the cruise. The cruise is going very well. Everyone is pulling together hard and we have some excellent results. The atmosphere on board is excellent. It has been lifted further by the upcoming birthdays of Peter and Mark. The delay in the cast on the following morning has allowed to properly mark the occasions! All are looking forward to getting up a little later than 5 am. I will probably just be out there early, out of habit and because the night sky here is absolutely beautiful.

VIDEO:
Dust on the horizon. Eric Achterberg
reports from the ship. |
The ship's officers and crew have been fantastic. Must also mention the engineers on the cruise. They are a cheerful bunch, and happily burst out in harmonious song. They are all making the cruise a success!
Dust! Finally!
We have had a very nice amount of dust in the atmosphere over the past 3 days. Just today it has diminished and the sky is clear again. However, during the last few days we were sailing in a haze with moist dusty air around us. The Meteorological Office in the UK (Dr Mark Harrison) is kindly providing information on the sources and trajectories of the wind and dust for us, and we have the back trajectory of the air/dust that reached the ship on January 18, when we were encountering high dust levels. The air travelled from the Sahara in a southwesterly direction with the trade winds and provided us with dust and clouds.

Saharan messenger! A Satellite image of Saharan dust falling on top of the RRS Discovery. Plymouth Marine Laboratory
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A satellite image of the top of the atmosphere (provided by Plymouth Marine Laboratory) is shown in the graph below and indicates that the dust plume was shrouded in the band of clouds. The aerosol collectors have been very busy over the past few days, and the filters have been orange-red from the Saharan dust. We will be using this dust in biological experiments, whereby we first rinse the nutrients off the filters and then provide this rinse water to bacteria and other microorganisms to observe how they react.
Drifter deployment
On Saturday afternoon we deployed a surface drifter that was positioned in a region where we had a significant dust deposition. As it drifts together with the waters that it is deployed in, the drifter acts as a marker of the patch of ocean water that received the dust over the last few days.
The drifter emits a signal to a satellite every 90 sec (Argos transmitter), and we reveive emails on the ship concerning the position of the drifter. We can then come back and observe the same patch of ocea. We will get back to it tomorrow and sample the waters there to see what has happened to the biology and chemistry after 3 days with very low dust inputs. Hopefully we can find the drifter again⌠it has a little light on top that will make spotting it easier at night.

Trapped on a filter! Saharan dust sampled on an air filter.
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Our heading now is back to the region of the Cape Verde Islands where we will sample the more productive waters. Following that we will head out west for a few days before making our way north to Tenerife via waters in the oligotrophic North Atlantic Gyre.
Hopefully plenty of dust to come still! Stay tuned!
Eric Achterberg
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A chain reaction
Tuesday 22 January 2008
Position: 12° 30 North, 32° 05 West
Weather: sunny, 25°C; Windspeed: force 5 Beaufort; Sea: moderate (2.5 m waves)
Water depth: 4460 m
by Laura Goldson, Marine Chemist (PML)
As I write this we are almost directly between Senegal and Brazil and, as you may expect, the weather is a little different from the UK winter at the moment. Despite the sunny day, the sea is quite rough. We are now steaming east towards Africa and the ship is moving around a fair bit. While seasickness is not a problem, the shipâs rolling continues to catch everyone out (watch the video diary '30 days' of a cruise in the Bermuda Triangle ).

VIDEO:
Waves climbing aboard?
All part of the trip. |
Most of the doors through the labs are water tight, to keep the water out of our working areas and living quarters if need be. That means big metal doors with high sills and thick rubber seals on them. More often than not, it is when I am mid doorway stepping over the sills and on one leg that the ship rolls under me and knocks me off balance! Quite amusing for anyone watching who is firmly planted on both feet! Besides this small problem, there is not too much on here to worry about. We are all very well looked after by the shipâs officers and crew, to such an extent that all we really have to worry about is our work.
I am beginning to think of the good ship Discovery almost as a second home since this is the fourth time I am sailing on her since November 2006. I am a marine chemist at Plymouth Marine Laboratory and my primary objective on this cruise is to measure the response of iodocarbons in seawater to Saharan dust inputs. Iodocarbons are iodine containing gases which are in much higher concentrations in seawater than the air above it and therefore easily move upwards from the surface ocean into the atmosphere. So! Well, they are important because, once in the atmosphere, they can impact our climate. The sun splits them up into iodine atoms which then react with ozone (a compound made up of three oxygen atoms which protects the Earth from the Sunâs harmful ultra-violet rays). The free iodine atom acts just like chloro-fluoro-carbons (more commonly know as CFCs) and steals the one oxygen atom from ozone breaking up the molecule to form new iodine-oxygen compounds (iodine oxides). In turn, iodine oxides act as the starting blocks for cloud formation which affects Earthâs albedo (remember Ruth Airsâ story of plankton producing itâs own clouds? well, this is similar!).

Laura mid-way through the water tight door into the deck lab where I do my analyses. |
The main link between iodocarbons and dust is again, phytoplankton. Iodocarbons have been shown to be produced by phytoplankton and therefore any increase in oceanic productivity due to a positive impact on the phytoplankton by dust, may also increase iodocarbon concentrations. If this were to happen, any potential impact on our climate would also increase.
I think all this gives me a pretty good reason (and believe me, one is required) to get up before the sun every morning at 5:25am to sample the first pre-dawn CTD Rosette cast and collect my seawater for those iodocarbon measurements!
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Bouncing off the bulkheads 
Wednesday 23 January 2008
Position: 12° 30 North, 30° 30West
Seastate: moderate: 2 m, Wind: Force 5
Water depth: 4620 m
by Tracy Lawson (University of Essex)
I felt very apprehensive as the RRS Discovery pulled away from Tenerife dock some 18 days ago, with my last window of opportunity to jump ashore slowly disappearing as the island faded into the distance.
This is my first time on board the Discovery or any other sea going vessel for that matter. I did not know how I would feel being on board a ship for a month; if I would suffer from sea sickness (I took some tablets just in case); never mind how I was going to carry out scientific experiments on a moving ship! (watch the video diary '30 days' of life and work at sea in the stormy Bermuda Triangle ).

VIDEO: Waves invading the deck, coffee dancing in the pot! It's all part of the game!
ipod version
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I am researcher from the University of Essex. Whilst on the cruise I am working with Steve Archer and Ruth Airs from Plymouth Marine Laboratory. We are looking at phytoplankton photo-inhibition (damage to photosynthetic apparatus) by excessive and ultra-violet light. (see Ruth Airs blog to see how stressed phytoplankton can produce their own atmospheric clouds to shade themselves from the sun!).
With the use of state-of-the-art instruments (Fast Repetition Rate Fluorometer and FIRe), we can determine determine how stressed the phytoplankton are and look for signs of photo-inhibition. We are also trying to identify recovery of damage to the photosynthetic apparatus through repair of the photosynthetic proteins (see also Anna Macey blog).
So what is it like living onboard Discovery? The aspect I found the most difficult to get used to was the continual movement of the ship. I couldnât sleep the first two nights onboard because I felt as if I was going to fall out of bed. Actually just yesterday morning, after getting out of bed, I only managed to walk half way across my cabin before I was suddenly thrown back in bed. I think it was a sign that 5.15am is too early in the morning to get up!

Tracy working on the FIRe machine (her favourite bandanna has now been lost at sea, blown by the strong winds somewhere in the Atlantic Ocean). |
It is a strange sensation sitting in your lab swivelling chair and suddenly being spun around, first to the left, followed by a sharp change of direction to the right as the ship rolls. It can also be very amusing seeing people walking down the corridor towards you and then suddenly disappearing into a door way only to return a few seconds later and continue their journey as if nothing had happened. And it is definitely a test of balance, and a real sense of achievement to navigate the chairs and tables in the galley (where we eat meals) and reach your breakfast table without spilling the poached eggs on the floor.
The labs aboard are just like our labs back at the university, only smaller. Despite working all the time we still have time to joke and laugh about life aboard. I didnât know many people before I came on board, but feel I have made some new friends.
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âRiders on the Stormâ 
Friday 25 January 2008
Position: 16° 10 North, 30° 30 West
Seastate: calm, Wind: Force 3-4 Beaufort, Temperature 25°C
Visibility: decreasing as we are in a big dust storm
Water depth: 5280 m
by Micha Rijkenberg, (NOCS)
It is 01:36 am and I just finished my day with some trace metal analysis (or should I say started my new day with some trace metal analysis!). After 20 days of hard work at sea, it becomes difficult to distinguish the days and when they start or finish. Fortunately most of my days start pretty late, at about 07:30 with breakfast instead of 05:30 as is the rule for most of my colleagues here on board the RRS Discovery.Â

"We are in a very nice big dust storm now. I will send you a nice satellite
picture of that tomorrow, together with the night sky tonight. Amazing!" -
Eric Achterberg, Chief Scientist
Image PML Remote Sensing Group.
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My task on the expedition is measuring iron in the dust and in the ocean (and we are now under an enourmous cloud of dust coming off Africa). As you might have already read, the trace metal iron has, due to its low concentrations in seawater, the potential to regulate the growth of microorganisms in many parts of the worldâs oceans (read more on Ocean Iron Fertilization, CO2 and climate). However, the concentrations of iron here in the equatorial North Atlantic are on average a little bit higher than in other open ocean environments. We think that the reason for that is the millions of tons of Saharan dust that is blown into the ocean. So, we came to investigate whether that is true.
The measurement of dissolved iron in seawater is a very precise job that needs constant attention. We do our job from a big and slightly rusty iron ship and we try to measure very, very low concentrations of iron. That means that we need all sorts of special equipment to prevent contamination of our samples. So just after breakfast, I and my colleagues Alex and Peter hurry to the trace metal clean container and dress up into our clean suits to prepare the arrival of freshly collected clean seawater samples. These seawater samples are collected with a special CTD Rosette that is totally made out of titanium and without any iron parts. The bottles of this titanium CTD are always cleaned with acid remove any contamination.

Luminol. Wikipedia |
In an industrial container, specially converted into a portable clean laboratory, we prepare the seawater samples for iron measurements (also for manganese and aluminum). We also collect seawater from the surface but from a distance from our iron ship. To do that, we use a heavy painted metal torpedo that glides alongside the ship through the water. The water is then pumped via a sort of plastic garden hose to the clean container were we sample it.
During our analysis, we concentrated the iron from seawater after which it is mixed with a chemical called luminol. The luminol starts to emit light in the presence of iron, so by measuring the light we can determine the exact concentration of iron. Every sample takes me at least 20 min, so in a working day that starts at 07:45h and ends at 23:00h I can measure on average about 25 samples! Since we collect about 25-30 samples a day, it is a constant race to catch up with work during the dust storm!
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Amino acids from the sky 
Sunday 27 January 2008
Position: 16° 33 North, 28° 48 West
Seastate: moderate to rough (2.5-3.5 m waves), Wind: Force 5-6 Beaufort
Water depth: 5270 m
by Pornsri Mingkwan, student at NOCS
This is my first research cruise and routinely, my day starts at 6.00 am every day with sampling the dawn cast of the CTD Rosette. I must say that working on the ship was quite difficult for the first few days, due to the movement of the ship, but I adapted and started enjoying my work. I love to see everyone enjoying themselves too despite the long hours and the hard work. I enjoy looking outward to the deep blue ocean. So far we have seen squid, sea turtles, flying fish and a shark. For the few days left on board I hope to see some more marine creatures, preferably dolphins or big whales!

Floating University!
Critical experiments cannot wait for the lab so the lab is brought to sea. Student Pornsri Mingkwan prepares samples for storage. |
As a student at the National Oceanography Centre, Southampton (NOCS), my interest is in amino acids. Believe it or not, rainwater and aerosols around me can contain amino acids, the building blocks of life! Where do they come from? Well, some of them have simply been ejected by the waves and spray from seawater into the atmosphere, but a lot them are carried over by the winds all the way from distant forest fires on land.
The reason we care about even the miniscule amounts of amino acids, is that just like the Saharan dust (read more), they supply rare nutrients to the phytoplankton in the ocean around me. As amino acids travel with the winds into the atmosphere, sunlight slowly but efficiently alters their molecular structure causing them to release simple nitrogen compounds that phytoplankton can assimilate. As a result, biomass burning can affect ecosystems many thousands of miles away!
This cruise is a fantastic oceanographic experience and I am having a great time working with the scientists, the officers and crew aboard.
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The end nears 
Thursday 31 January 2008
Position: 25° 04 North, 28° 28 West
Wind: Force 7, Seastate rough (3.5 m waves)
Water depth: 5585 m
by Matt Patey (NOCS)
Only a few days left before we return to Tenerife. It has been a busy month and the fatigue is showing. I, for one, feel particularly rough round the edges after I prise myself out of bed in the mornings; a difficult task at the best of times! Despite the fatigue, the mood is positive and the science has gone very well. With the end nearing, talk has now turned to what we are all going to do once we set foot upon dry land (something none of us have seen much of since the 5th January).

Sun haze during dust storm.
Matt Patey
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Fresh fruit and salads are high on some peopleâs list of priorities. While the galley staff on board are still doing an incredible job of knocking out delicious meals three times a day, some of the more perishable foods are gone and the fruit bowl now contains only apples. Personally, having been confined to the ship for a month, I am looking forward to simply having the freedom to walk anywhere I choose. Higher up on my list of priorities, however, are several extremely long lie-ins.
This is my fourth time at sea and each time I am taken aback by the wildness and remoteness of my surroundings. I find that I use my imagination more than on other occasions. Every day I look out the port hole, there is water as far as the eye can see in every direction. I feel like we really could be anywhere in the ocean and I wouldn't know any different. Only the GPS displays around the ship tell me that we are presently at 25° North, 28° West (which still doesn't mean very much to me, but at least I can look it up on a map!). The same displays also inform me that the sea-bed is a staggering 5585 meters beneath my feet! I find this very difficult to visualize.

VIDEO report from Chief Scientist Eric Achterberg aboard the RRS Discovery. |
The reason I am here is to study the dissolution of Saharan dust in seawater. As you have probably heard already, the huge clouds of Saharan dust that are flying high above the Discovery right now, can act as a source of nutrients (and trace metals) vital for phytoplankton growth in the open ocean. My work focuses on measuring which nutrients are coming from the dust and how much of them dissolve in the ocean. We have been very fortunate this cruise, and collected a considerable amount of dust on our air filters!
I then use this dust to run my experiments, and together with the biologists on board we also do incubation experiments to see under controlled conditions whether dust is beneficial to phytoplankton, and how much. After all the experiments are done, I often take a cup of tea to the bow |